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Description
In the proposed study a novel 3-layered and composite biopolymer scaffold of customizable porous structure and preset biological activity will be prepared by the freeze-drying method. The scaffold will be composed of three functional layers: 1st one made of collagen, 2nd of polymer mixtures based on sodium hyaluronate and its mixtures with other polymers enriched with the selected active compounds; 3rd the nano-monolayer of titanium dioxide serving as functional coating with bactericidal and bacteriostatic properties and reducing the hydrophilicity. It is expected that the prepared structure will be universal for different applications and easily adjusted to the targeted active compound. The last part of the project anticipates the elaboration of the variant of scaffold directed to the use in skin cell engineering (prototype). It is expected that the final construct and its 3-layered system will play crucial role in each stage of wound healing process.
Summary of project results
The project was needed to address the challenges in guided bone and tissue regeneration treatments (GBTR) and skin cell engineering by developing a novel 3-layered composite biopolymer scaffold with porous structures and biological activity.
Work done within the project:
- the focus was on creating a collagen-based freeze-dried foam-like structure. This layer was essential for providing mechanical stability and durability while mimicking the extracellular matrix (ECM).
- a hyaluronic acid-based freeze-dried foam-like structure was developed, enriched with active compounds such as elastin, sodium alginate, hydroxyapatite, and selected natural extracts.
- a prototype 3-layered composite construct was created aimed at skin tissue engineering applications. The prototype maintained biocompatibility and exhibited relatively increased stability under physiological conditions, appropriate physicochemical characteristics, and ensured biocompatibility.
The main results of the project included the successful creation of a 3-layered composite biopolymer scaffold. This includes a collagen layer for mechanical stability, a hyaluron layer for cell growth and nutrient support, and a titanium dioxide nano-monolayer for decrease of the solubility in biological conditions. The project, if continued, could made a significant difference for its end beneficiaries, including patients requiring bone and tissue regeneration, by providing a more effective and reliable solution for tissue engineering. This scaffold could assist in each stage of the tissue development process, ensuring proper performance and enhancing the effectiveness of treatments.