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Description
1. Development of micromachined ionization interfaces allowing highly sensitive mass spectrometry analysis of pre-separated biochemical compounds.
2. Development of micromachined separation system utilizing the own patented principle of recycling chromatography in which the sample passes repeatedly though the (micro)separation column leading to a significant increase of resolution of its components.
3. Development of methodology for coupling of conventional microcolumn-based separation and on-a-chip separation techniques to mass spectrometer through the micromachined interfaces.
4. Demonstration of innovation potential of the developed technologies by showcasing two demonstrators targeting high impact applications relevant for life sciences and pharma industries.
Summary of project results
The μBIOSEP project aimed to address the need for more efficient and miniaturized systems for sensitive bioanalysis, of challenging but scientifically or clinically significant biological samples, such as human plasma. Traditional bioanalytical methods can be time-consuming, expensive, and require large sample volumes. The project sought to overcome these limitations by developing innovative microfluidic and lab-on-a-chip technologies.
The project focused on the research and development of miniaturized interfaces for electrospray ionization and a microfluidic device enabling their coupling with commercial mass spectrometers., which functionality was experimentally verified. Furthermore, the concept of miniaturized valves was investigated using computer simulations. The microvalves represent a key component of the envisaged system for the separation of short fragments of the circulating DNA that can be used for cancer diagnosis from blood plasma samples. A study demonstrating the applicability of the developed device for the analysis of complex samples from the life sciences and pharmaceutical industries was also prepared.
The project achieved functional samples, verified technologies, a utility model and many other dissemination results related to the developmnet of miniaturized bioanalysis system. The system shall be applied for analyzing short fragments of circulating DNA that can be used for the early diagnosis of cancer using the liquid biopsy technique. The outcomes benefit researchers in fields like medicine, biotechnology, and environmental science by providing them with more efficient and accessible tools for bioanalysis. This could lead to advancements in diagnostics, drug discovery, and other areas.
Summary of bilateral results
The bilateral cooperation, mutual research and then the development involved in the project was carried out with a high degree of expertise, professional and meaningful collaboration, and efficiency. The established bilateral cooperation will continue.